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. 2011 Oct 14;10:129. doi: 10.1186/1476-4598-10-129

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Copyright ©2011 Ummanni et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Effects of overexpression of UCHL1 on the expression of cell cycle related proteins. (A) Representative Western blots against UCHL1, p27Kip1, Cyclin A and β-actin, as a house keeping protein, demonstrated that UCHL1 induces accumulation of p27Kip1 and reduction of Cyclin A expression in LNCaP cells. (B-C) Quantitative RTPCR revealed no statistically significant change of p27Kip1 and Cyclin A mRNA level in UCHL1 positive cells. The results are indicated as mean ± SD of 3 independent experiments. (D) Protein levels of Rb, pRb, Cyclin E, Cyclin D and β-actin were detected by Western blotting analyses using corresponding antibodies in UCHL1 overexpressing LNCaP cells. (E) Quantitative RTPCR revealed no statistically significant change of Cyclin D mRNA level in UCHL1 positive cells. The results are indicated as mean ± SD of 3 independent experiments.