Table 5.

qRT-PCR to monitor gene expression after introducing a point mutation upstream of spr1021 (G-29T) and spr1887 (G-46T) in the S. pneumoniae 1974 and T-7 lines.

Strains/transformants	Mean 1974T/1974WT spr1021 expression ratioa, b, c	Mean 1974T/1974WT spr1887 expression ratioa, b, c
T-1974spr1021	3.6 (0.4)	1.2 (0.2)
T-7spr1021	3.4 (0.4)	1.2 (0.2)
T-7spr1021, spr1887	3.3 (0.5)	12.9 (0.2)
1974	1 (0.2)	1 (0.2)

^aT (transformant); WT (wild-type)

^bValues represent an average of three independent experiments.

^cThe standard deviation is indicated in parentheses.