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. 2011 Sep 23;26(12):3249–3263. doi: 10.1093/humrep/der317

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© The Author 2011. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited

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NaCN inactivates ETC-dependent oxygen consumption in human spermatozoa. Respiration rates for capacitated spermatozoa incubated in the presence of 5 mM glucose and a combination of 5 mM glucose and 5 mM pyruvate. The data are presented as relative to respiration capacity during glucose + pyruvate treatment (mean ± SEM from three separate experiments, n= 3). (A) Relative basal (basal), total (FCCP) and ETC-independent (NaCN) respiration capacity is shown. (B) NaCN blocks respiration during glucose (○) and glucose + pyruvate (▪) administration.