Figure 7.

The reduced levels of ATP and motility in human spermatozoa treated with mitochondrial inhibitors can be rescued by methylene blue. Spermatzoa were incubated under capacitating conditions supplemented with 5 mM glucose and 10 mM NaCN, 10 µM rotenone or 1 µM antimycin A for 120 min in the absence (black bars) and presence of 5 mM pyruvate (grey bars) or 50 µM methylene blue (white bars). (A) ATP concentrations measured by luminescence. (B–E) Sperm motility evaluated by CASA. The data are presented as mean ± SEM from three separate experiments (A; n=9, B–E; n= 6).