Restriction analysis of genomic DNA and of pCP29 isolated from E. coli λ pir and F. psychrophilum THCO2-90. Genomic or plasmid DNA was obtained as described in the text and was digested with different restriction enzymes. Lanes 2 to 5, F. psychrophilum genomic DNA; lanes 6 to 9, pCP29 isolated from F. psychrophilum; lanes 10 to 13, pCP29 isolated from E. coli. DNA was digested with BamHI (lanes 3, 7, and 11), MboI (lanes 4, 8, and 12), or Sau3AI (lanes 5, 9, and 13). No restriction enzymes were added to the samples in lanes 2, 6, and 10. Lane 1 contained λ DNA digested with PstI as a reference.