TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description | Source or reference |
|---|---|---|
| Bacterial strains | ||
| E. coli | ||
| S17-1 λ pir | λpir hsdR pro thi; chromosomal integrated RP4-2 Tc::Mu Km::Tn7 | 25 |
| BW19851 | RP4-2tet::Mu-1kan::Tn7 integrant; ΔuidA::pir+recA1 hsdR17 creB510 endA1 zbf-5 thi | 17 |
| F. psychrophilum | ||
| ATCC 49418 | Wild type | ATCCa |
| ATCC 49510 | Wild type | ATCC |
| ATCC 49511 | Wild type | ATCC |
| D12 | Wild type | 15 |
| OSU SH3-81 | Wild type | 4 |
| OSU THCO2-90 | Wild type | 4 |
| OSU THCO4-90 | Wild type | 4 |
| OSU Ch8-80 | Wild type | 4 |
| FPC 830 | Wild type | 4 |
| Plasmidsb | ||
| pCP29 | ColE1 ori; (pCP1 ori); Apr (Cfr Emr); E. coli-F. psychrophilum shuttle plasmid | 11 |
| pCP23 | ColE1 ori (pCP1 ori); Apr (Tcr); E. coli-F. psychrophilum shuttle plasmid | 1 |
| pIVET8 | pir-dependent oriR6K; Mob+ Apr | 14 |
| pCP23-β | 6-kb SphI fragment of pIVET8 containing lacZY genes cloned in pCP23 | This study |
| pEP4351 | pir-requiring R6K oriV; RP4 oriT; Cmr Tcr (Emr); vector for Tn4351 transfer | 5 |
a
ATCC, American Type Culture Collection, Rockville, Md.
b
Antibiotic resistance phenotypes: ampicillin, Apr; cefoxitin, Cfr; chloramphenicol, Cmr; erythromycin, Emr; tetracycline, Tcr. Antiobiotic resistance phenotypes and other features listed in parentheses are those expressed by F. psychrophilum but not by E. coli.