Figure 3.
Two-way FACS separation segregates non-human primate spermatogonia from inoculated testis cell suspensions into the CD90+/CD45− fraction. (A) Unsorted prepubertal non-human primate testis cells and the sorted (B) CD90+/CD45− and (C) CD90−/CD45− fractions from inoculated cell suspensions were stained for the pan germ cell marker VASA (green) and counterstained with DAPI (blue). Scale bar = 50 µm. (D) Quantification of percentage of cells in each unsorted and sorted fraction that were VASA+ from each replicate experiment and the calculated VASA+ spermatogonial number is shown for each fraction. The percent recovery of VASA+ spermatogonia in the CD90+/CD45− fraction is presented relative to the starting number in the Unsorted fraction. Statistically significant differences (ANOVA on nested generalized linear mixed-effects models) in the VASA+ percentage between the Unsorted and either CD90+/CD45− (P = 2.2 × 10−16; *) or CD90−/CD45− (P = 6.17 × 10−8, **) are noted in superscript.