FIG. 2.

Batch cultures of C. glutamicum strains pLPIotsBA01 (A) and pLPIotsBA02 (B) in DMCG II (see Materials and Methods). The temporal profiles of glucose (□), biomass (○), and trehalose (•) are shown. The culture medium used was supplemented with chloramphenicol at 20 mg/liter to avoid plasmid loss, and IPTG (inducer) was added at 4 h of cultivation.