TABLE 1.
Bacterial strains and plasmids used in this work
| Strain or plasmid | Genotype and/or description | Source or reference |
|---|---|---|
| Plasmids | ||
| pGEM-T | Ampr | Promega |
| pXMJ-19 | Cfr | 20 |
| pLPIotsBA00 | otsBA Ampr | This study |
| pLPIotsBA01 | ptac otsBA (direct insert) Cfr | This study |
| pLPIotsBA02 | ptac otsBA (inverted insert) Cfr | This study |
| Strains | ||
| E. coli DH5α-mcr | supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA mcrA Δ(mrr-hsdRMS-mcrBC) | 16 |
| E. coli/pLPIotsBA00 | otsBA Ampr | This study |
| E. coli/pLPIotsBA01 | ptac otsBA (direct insert) Cfr | This study |
| E. coli/pLPIotsBA02 | ptac otsBA (inverted insert) Cfr | This study |
| C. glutamicum ΔtreS | ΔtreS derivative of C. glutamicum 13032 | 45 |
| C. glutamicum/pLPIotsBA01 | ΔtreS ptac otsBA (direct insert) Cfr | This study |
| C. glutamicum/pLPIotsBA02 | ΔtreS ptac otsBA (inverted insert) Cfr | This study |