TABLE 2.

Specific activities of some enzymes of the central metabolism in crude extracts from C. glutamicum after induction with 1 mM IPTG^b

Enzyme	Sp act (pkat/mg of protein)a
	ΔtreS	ΔtreS pLPIotsBA01	ΔtreS pLPIotsBA02
G-6-phosphate-gluconate- 6-phosphate dehydrogenase	192	39	173
Phosphoglucoisomerase	12,524	17,891	14,038
Pyruvate kinase	7,119	5,716	6,014
Isocitrate dehydrogenase	37,124	31,303	38,016
Malate dehydrogenase	22,018	22,198	21,629

^aTabulated activities are the means of at least two independent measurements, and the standard deviation was always less than 10%.

^bExtracts prepared from cells grown in shake flasks containing DMCG I medium and induced for 2h with 1 mM IPTG. The inducer was added when the culture reached an optical density at 600 nm of 0.3 to 0.4.