Figure 5.

ACS activity in crude extracts from 3-d-old, etiolated wild-type (WT), eto1, and eto3 mutant seedlings. The enzyme was assayed by incubating crude extracts in buffer with or without AdoMet, and then measuring the amount of ACC formed by converting it to ethylene (see Methods). The activity was calculated by subtracting the amount of ethylene produced in the absence of added AdoMet and then normalizing to the protein concentration of each sample. Values are means (±sd) of three replicates.