TABLE 1.
Bacterial strains and plasmids used in this study
| Plasmid or strain | Characteristic(s) | Reference or origin |
|---|---|---|
| Plasmids | ||
| pBluescript | High-copy-number plasmid | Stratagene |
| pACYC184 | Medium-copy-number plasmid | 6 |
| pSYM1 | pBluescript containing 5.6-kb fragment from E. aerogenes | This study |
| pSYM2 | pSYM1 with 0.5-kb deletion between BamHI and XhoI sites in E. aerogenes fragment | This study |
| pSYM3 | pTrcHisB opdA | This study |
| pSYM4 | Expression cassette from pSYM3 cloned into pACYC184 | This study |
| pSYM5 | pSYM4 with trp promoter mutated to lac promoter | This study |
| Strains | ||
| Enterobacter aerogenes | Field strain of Enterobacter aerogenes #251 | 18 |
| DH10B | Escherichia coli strain | Gibco BRL |
| GpdQ− | DH10B with pBluescript | This study |
| GpdQ+ | DH10B with pSYM1 | This study |
| GpdQ+ UgpB− | DH10B with pSYM2 | This study |
| GpdQ− OpdA+ | DH10B with pBluescript and pSYM5 | This study |
| GpdQ+ OpdA− | DH10B with pSYM1 with pACYC184 | This study |
| GpdQ+ OpdA+ | DH10B with pSYM1 with pSYM5 | This study |