TABLE 2.
Substrate | Specific activity (μmol/min/mol of GpdQ)
|
|
---|---|---|
pH 5 | pH 8 | |
pNPP | 9.7 × 102 | 1.8 × 101 |
bpNPP | 1.5 × 104 | 1.0 × 104 |
DMP | 2.4 × 102 | 1.5 × 102 |
GPE | 5.5 × 104 | 2.6 × 104 |
Demeton | 2.6 × 103 | 1.2 × 103 |
Paraoxon | 3.1 × 101 | 1.2 × 102 |
Assays were conducted in 20 mM sodium acetate, pH 5.0, and 20 mM HEPES, pH 8.0, at room temperature.