Table 1.

Antibodies used and immunohistchemical result.

Antibody	Clone	Dilution	Source	Stroma around the micropapillary clusters		Tumor cells
						in SMP		outside SMP
				Case1	Case2	Case1	Case2	Case1	Case2
MUC1	Ma695	1:100	Novocastra	0	0	2+	2+	1+	2+
EMA	E29	Pre-diluted	Cell Marque	0	0	2+	2+	2+	2+
E-cadherin	NCH38D	1:100	DakoCytomation	0	0	2+	2+	2+	2+
CK	AE1/AE3	Pre-diluted	Nichirei	0	0	2+	2+	2+	2+
TTF-1	8G7G3/1	1:100	DakoCytomation	0	0	1+	2+	2+	2+
SP-A	PE10	1:100	Dako	0	0	1+	0	2+	2+
D2-40	D2-40	Pre-diluted	Nichirei	0	0	0	0	0	0
CD34	Nu-4A1	Pre-diluted	Nichirei	0	0	0	0	0	0
FactorVIII	polyclonal	1:200	DAKO	0	0	0	0	0	0

EMA, epithelial membrane antigen; CK, cytokeratin; TTF-1, thyroid transcription factor-1; SP-A, surfactant protein A.

Novocastra, Newcastle Upon Tyne, UK; Cell Marque, Hot springs, AR, USA; DakoCytomation, Carpinteria, CA, USA; Dako, Kyoto, Japan; Nichirei, Tokyo, Japan; DAKO, Glostrup, Denmark.

0, no positive cells; 1+, positive tumor cells less than 10%; 2+, 10% or more.