FIG. 1.
Schematic representation of the positions of the primers used to analyze LEE insertion. Insertion of LEE at selC (A), pheU (B), and pheV (C) tRNA sites was analyzed. LEEE2348/69 and LEEEDL933 are located at 82 min on the E. coli genome. LEE83/39 and LEE84/110-1 are located at 94 and 67 min, respectively, on the E. coli genome. The positions of the probes used for Southern hybridization (PFGE analysis) are indicated by gray boxes. Nucleotide sequences are available at the GenBank database as follows: LEEE2348/69, AF022236; LEEEDL933, AF071034; LEE83/39, AF453441; LEE84/110-1, AF453442; selC, AE000443; pheU, AE000486; pheV, AE000486.