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. 2004 Jan;70(1):61–68. doi: 10.1128/AEM.70.1.61-68.2004

FIG. 2.

FIG. 2.

Determination of the integration site of the LEE by PFGE and Southern hybridization. (A) PFGE patterns of chromosomal DNA digested with I-CeuI. (B) Southern hybridization with the eae1 probe specific to the conserved 5′ end of eae. Lanes: 1, E. coli DH5α; 2, RDEC-I; 3, E2348/69; 4, EDL933; 5, NV100; 6, NV163; 7, NV169; 8, NV220; 9, NV258; 10, NV270; 11, NV2; 12, NV10; 13, NV19; 14, NV92, 15, NV95; 16, NV281, 17, Saccharomyces cerevisiae DNA size standard (Bio-Rad). The fragment sizes (in kilobases) of the E. coli K12 DNA digested with I-CeuI are shown at the left.