TABLE 3.
Analysis of LEE insertion site
| Strain | PCR analysis of the selC sitea
|
PCR analysis of the pheU site
|
PCR analysis of the pheV site
|
Gene present by PFGE analysisd | |||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| selC site | LEE right side | LEE left side | CP4-int | pheU site | LEE right side | LEE left sideb | int-phe | pheV site | int-phec | ||
| NV100 | I | NR | NR | NR | D | − | − | + | D | + | pheV |
| NV163 | I | NR | NR | NR | D | − | − | + | D | + | pheV or pheU |
| NV169 | I | NR | NR | NR | I | NR | NR | − | D | + (2.4 kb) | pheV |
| NV220 | I | NR | NR | NR | D | − | − | − | D | + (2.4 kb) | pheV |
| NV258 | I | NR | NR | NR | D | − | − | + | D | + | pheV |
| NV270 | I | NR | NR | NR | I | NR | NR | − | D | + | pheV |
| NV2 | D | + | + | − | I | NR | NR | − | I | NR | selC |
| NV10 | D | − | − | + | D | + | + (2.3 kb) | + | D | + | pheU |
| NV19 | D | − | − | − | I | NR | NR | − | D | + | pheV |
| NV92 | D | − | + | + | D | − | − | + | D | + | selC |
| NV95 | D | − | + | − | I | NR | NR | − | D | + | selC |
| NV281 | D | − | − | + | D | + | + (6.0 kb) | + | D | + | ND |
a
PCRs were performed with the primers shown in Fig. 1. I, intact site; D, disrupted site; NR, not relevant due to an intact selC, pheU, or pheV site; ND, not determined; +, amplification; −, no amplification.
b
Amplicon sizes distinct from that obtained with the RDEC-I reference strain are indicated in parentheses.
c
Amplicon sizes distinct from that obtained with the 84110-1 reference strain are indicated in parentheses.
d
tRNA locus present in the DNA fragment containing the LEE.