TABLE 3.

Clones in the alr complementation library that are capable of growth in the presence of a maximum concentration of 10, 100, or 300 μg of d-cyloserine/ml, without the addition of d-alanine

[d-Cycloserine] (μg/ml)	Clone name	Insert start coordinateb	Estimated insert size (kb)	ORF in insertc	Function of homologue(s)
10	pNZ7120-csl1	1037318	1.7	lp_1141 (purK2)	Phosphoribosylaminoimidazole carboxylase, ATPase subunit
10	pNZ7120-csl2	1216019	2.0	lp_1320	Conserved hypothetical protein
10	pNZ7120-csl3	1000151	2.5	lp_1101 (ldhl2)	Lactate dehydrogenase
10	pNZ7120-csl4	2617565	2.5	lp_2937	Transcription regulator
10	pNZ7120-csl5a	1309715	3.0	lp_1430	Glycosyltransferase
10	pNZ7120-csl6a	1904621	2.6	lp_2109 (urvC)	Excinuclease ABC, subunit C
10	pNZ7120-csl7	416640	2.5	lp_0464	Zinc/iron ABC transporter
10	pNZ7120-csl8a	3008044	3.0	lp_3392	Transport protein
100	pNZ7120-csm1	470883	1.7	lp_0525 (kupI)	Potassium uptake protein
100	pNZ7120-csm2	3035271	1.7	lp_3418 (pck)	Phosphoenolpyruvate carboxykinase
100	pNZ7120-csm3	1038952	1.2	lp_1144 (pcrA)	ATP-dependent DNA helicase
100	pNZ7120-csm4	3308262	1.6	lp_3688a (rpmH)	Ribosomal protein L34
100	pNZ7120-csm5	2948260	1.6	lp_3312	Conserved hypothetical protein
100	pNZ7120-csm6	2373288	1.8	lp_2671	Integral membrane protein
100	pNZ7120-csm7a	2019577	2	lp_2231a	Hypothetical protein
100	pNZ7120-csm8a	2855464	1.5	lp_3204 (nupC)	Nucleoside transport protein
100	pNZ7120-csm9a	1454904	3	lp_1599 (folD)	Methylenetetrahydrofolate dehydrogenase and cyclohydrolase
100	pNZ7120-csm10	2039898	2.5	lp_2256 (ccpA)	Catabolite control protein A
100	pNZ7120-csm11	90985	2.8	lp_0101	Cobalt ABC transporter component
300	pNZ7120-csh1	2668545	1.7	lp_3000	ABC transporter, ATP-binding and permease protein
300	pNZ7120-csh2a	3015121	2.5	lp_3399 (cpd)	2′,3′-Cyclic-nucleotide 2′-phosphodiesterase precursor
300	pNZ7120-csh3a	2483803	2.5	lp_2793	Hypothetical protein
300	pNZ7120-csh4	1383808	2	lp_1512 (dnaB)	Replication initiation and membrane attachment protein DnaB
300	pNZ7120-csh5	676236	2.2	lp_0739 (secA)	Preprotein translocase, SecA subunit
300	pNZ7120-csh6	2990240	0.5	lp_3363 (copB)	Copper-transporting ATPase
300	pNZ7120-csh7	493323	1.8	lp_0542	Conserved hypothetical protein
300	pNZ7120-csh8	2326766	2.4	lp_2608	Na+/H+ antiporter

^aClones used for Western blot analysis (Fig. 2).

^bInsert start coordinate indicates the first base pair of the L. plantarum genome sequence in the insert of the pNZ7120 derivatives directly upstream of alr.

^cGene names are indicated between brackets if present in the present annotation of the L. plantarum WCFS1 genome (26).