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The effect of temperature on the activity of free and immobilized lipase. Activity was measured at 37°C after treatment for 1 hour in phosphate buffer (10 mM, pH 7.0) at the temperatures indicated. The PD-MNPs were prepared using 2.5 mg/ml initial dopamine and lipase immobilization performed using 5 mg/ml initial lipase and 10 mg/ml PD-MNPs concentration.
