Skip to main content
. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: Biochemistry. 2011 Sep 22;50(41):8853–8861. doi: 10.1021/bi200981a

Figure 4.

Figure 4

Reciprocal mutations leading to conversion between IPC and SM/EPC synthase activity in TbSLS1 and TbSLS3. A, SDS-PAGE analysis of expression for the different TbSLS variants with mutations indicated. B, thin-layer chromatography analysis of products given by the different TbSLS variants using NBD-C6-CM as substrate. Three mutations convert TbSLS1 from an IPC synthase to a predominantly bifunctional SM/EPC synthase. The reciprocal mutations convert TbSLS3 from a bifunctional SM/EPC synthase into an IPC synthase.