Table III.

Analysis of the curve-fitting procedures for kinetics of fluorescence quenching in LHCII and chloroplasts

Sample	Eq	Norm	a1		a2		k1		k2
			cv	dep	cv	dep	cv	dep	cv	dep
		relative units	relative units		relative units		relative units		relative units
LHCIIb	1	13.1  ± 2.3	3.5  ± 1.0	0.7  ± 0.2	2.1  ± 0.2	0.7  ± 0.1	6.1  ± 0.8	0.8  ± 0.1
	2	8.3  ± 2.1	2.5  ± 0.5	0.9  ± 0.06	3.1  ± 0.2	0.9  ± 0.1	11.0  ± 1.6	0.87  ± 0.1	6.0  ± 0.7	0.87  ± 0.12
	3	5.1  ± 1.4	2.2  ± 0.3	0.6  ± 0.1	1.2  ± 0.2	0.7  ± 0.07	3.6  ± 0.6	0.8  ± 0.1
	4	3.9  ± 1.6	1.9  ± 0.4	0.8  ± 0.2	2.1  ± 0.2	0.8  ± 0.12	7.1  ± 0.8	0.85  ± 0.1	2.5  ± 0.7	0.95  ± 0.05
Chloroplast	1	6.3  ± 1.0	9.0  ± 2.2	0.4  ± 0.1	5.1  ± 1.3	0.3  ± 0.1	7.1  ± 1.1	0.4  ± 0.2
	2	4.1  ± 0.6	8.1  ± 1.5	0.3  ± 0.1	7.2  ± 2.4	0.9  ± 0.3	6.1  ± 1.2	0.4  ± 0.2	14.0  ± 2	0.86  ± 0.2
	3	3.0  ± 0.5	4.0  ± 1.3	0.06  ± 0.02	0.8  ± 0.2	0.3  ± 0.1	5.0  ± 0.6	0.4  ± 0.2
	4	2.9  ± 0.5	5.3  ± 1.4	0.4  ± 0.01	2.0  ± 1.2	0.9  ± 0.2	9.0  ± 1.1	0.7  ± 0.2	6.3  ± 2.1	0.87  ± 0.3
CP26	1	5.0  ± 0.3	7.3  ± 0.3	0.8  ± 0.1	10.3  ± 0.8	0.4  ± 0.2	7.0  ± 0.6	0.6  ± 0.2
	2	3.0  ± 0.1	6.3  ± 0.6	0.8  ± 0.1	5.6  ± 0.3	0.9  ± 0.1	13.2  ± 1.4	0.9  ± 0.1	5.3  ± 0.9	0.9  ± 0.1
	3	3.0  ± 0.1	6.3  ± 0.5	0	2.1  ± 0.3	0.2  ± 0.06	1.7  ± 0.5	0.4  ± 0.06
	4	2.2  ± 0.06	4.0  ± 0.3	0.4  ± 0.03	2.0  ± 0.2	0.7  ± 0.1	2.1  ± 0.6	0.8  ± 0.1	2.0  ± 0.8	0.7  ± 0.1

Fluorescence quenching curves were fitted to four different kinetic models: Eq 1 − Ft = a1.exp(−k1.t) + a2; Eq 2 − Ft = a1.exp(−k1.t) + a2.exp(−k2.t); Eq 3 − Ft = 1/(k1.t + 1/a2) + a2; Eq 4 − Ft = 1/(k1.t + 1/a2) + 1/(k2.t + 1/a2). cv, Coefficient of variation; dep, parameter dependency. Data were obtained from the analysis of three replicate quenching curves.