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. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: Chem Res Toxicol. 2006 Apr;19(4):531–538. doi: 10.1021/tx050348d

Figure 4.

Figure 4

HPLC-ESI+-MS/MS method validation for O6-Me-G using isotope dilution with O6-CD3-G. Known amounts of synthetic DNA duplex containing a single O6-Me-G residue (5'-GTA GTT GGA GCT GGT GGC [O6-Me-G] TA GGC AAG AGT-3') (0.050 to 1 pmol) were mixed with 1 pmol of the corresponding unmethylated duplex and 250 fmol of denatured AGT protein, followed by mild acid hydrolysis, addition of O6-CD3-G internal standard, and HPLC-ESI+-MS/MS analysis. O6-Me-G was quantitated from the HPLC-ESI+-MS/MS peak areas of O6-Me-G and O6-CD3-G.