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. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: Chem Res Toxicol. 2006 Apr;19(4):531–538. doi: 10.1021/tx050348d

Figure 6.

Figure 6

Time course of O6-Me-G repair within a DNA duplex derived from K-ras gene in the presence of different amounts of AGT. Synthetic DNA duplex 5'-GTA GTT GGA GCT [O6-Me-G] GT GGC GTA GGC AAG AGT-3', (+ complement, 500 fmol) was incubated with 50, 200, 428 or 856 fmol of human recombinant AGT protein, and the reactions were terminated at different time points by the addition of HCl. The samples were acid hydrolyzed to release purines, followed by the addition of O6-CD3-G internal standard and HPLC-ESI+-MS/MS analysis of O6-Me-G.