Skip to main content
. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: Chem Res Toxicol. 2006 Apr;19(4):531–538. doi: 10.1021/tx050348d

Figure 7.

Figure 7

Time course for AGT repair of O6-Me-G located at the first and second position of K-ras codon 12. Synthetic DNA duplexes containing a single O6-Me-G residue at either G6 or G7 of the (+) strand: 5'-G1TA G2TT G3G4A G5CT G6G7T G8G9C G10TA G11G12C AAG13 AG14T-3' (500 fmol) were combined with human recombinant AGT (450 fmol). The reactions were terminated at different times by the addition of HCl, and the samples were acid hydrolyzed to release purine bases. Unrepaired O6-Me-G was quantified by HPLC-ESI+-MS/MS using O6- CD3-G as an internal standard.