FIG. 5.

Detecting rearrangements in cell-lines with complex rearrangements. Sequencing results confirming the breakpoint locations in CEM (a), MOLT4 (b), and A549 (c) cell-lines. The presence of multiple forward primers in CEM requires the use of alternating multiplexing. (d) Array results for the A549 cell line. Note that the array not only captures the left and right breakpoints, but also an inserted inversion. The remainder of the spots correspond to nonspecific background signals (corresponding to repeat locations) present across runs.