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. Author manuscript; available in PMC: 2011 Nov 10.
Published in final edited form as: Exp Eye Res. 2007 Aug 29;85(6):799–805. doi: 10.1016/j.exer.2007.08.015

Figure 1.

Figure 1

Trypan blue staining (dead or dying cells) of cultured HCEC after 5 h exposure to ~1 × 106 cfu strain PA103ΔexoUexoT::Tc (PA103ΔUT) complemented with empty vector pUCP18 (A), pUCPexoU (B), the phospholipase-inactive mutant pUCPexoUD344A (C) or media (KBM) only (D). ExoU-complemented bacteria caused a typical pattern of cell death. Bacteria complemented with phospholipase-inactive ExoU caused little or no cell death similar to vector and media only controls. Images were adjusted using Adobe Photoshop to equate brightness and contrast.