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Apoptosis analysis by FACS using Annexin V-FITC stain assay. MCF7 and U87MG cells were incubated with 1 μM of DOX formulations for 24 h. To compare apoptosis, FITC-conjugated annexin binding to phosphatidyl serine, exposed to the outer leaflet, on treatment with DOX formulations was measured by FACS.
