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. 2011 Nov 10;7(11):e1002353. doi: 10.1371/journal.ppat.1002353

Figure 6. Transient silencing of Avr3b impaired P. sojae virulence on soybean.

Figure 6

In vitro synthesized Avr3b dsRNA was introduced into P. sojae P6497, generating 16 lines. Avr3b transient transformants and recipient strain P6497 were examined for Avr3b transcript levels and virulence. RNA samples were extracted at the germinating cyst stage. (A) Avr3b transcript levels were determined by Real-time PCR using P. sojae actin transcripts as a reference and then normalized to the wildtype (P6497). Bars represent standard errors from 2 independent RNA isolation and Real-time PCR replicates. Letters indicated above each column represent statistical significance based on Duncan's multiple range test (p <0.01). (B) The disease lesion lengths in etiolated soybean hypocotyls (susceptible cultivar Williams) infected with zoospores of Avr3b transient transformants and P6497 at 36 hpi. Means and standard errors from at least 10 measurements are shown. Two statistical tests was used. The letters indicated above each column represent statistical significance based on Duncan's multiple range test (p <0.01). The asterisk indicates statistical significance based on student's t-test (p <0.01).