Figure 3.
Expression of apyrase in pea and in transgenic lines. A, Immunoblot analysis of subcellular fractions from etiolated pea plants. Lane 1, Cytoplasm; lane 2, purified plasma membrane; lane 3, purified nuclei; and lane 4, preimmune control of nuclei. B, Top, The total Pi accumulated in the shoots of the wild type (Wt) and three independent transformants in an 18-h 32P-uptake assay tested at 2 mm Pi. Bottom, A corresponding immunoblot performed on equal amounts of protein isolated from the ECM of 3-week-old wild-type Arabidopsis and the psNTP9 transgenics. C, Assay of ATPase activity in the ECM fraction of OE1.