TABLE 2.
Serinol, dihydrorhizobitoxine, and rhizobitoxine production by B. elkanii USDA94 and rhizobitoxine-deficient mutants in culture and in nodules of G. max cv. Lee
| B. elkanii strain | Production in culture supernatant (μM)b
|
Production in nodule extract (μmol/g of fresh wt)b
|
||||
|---|---|---|---|---|---|---|
| Serinol | DRTa | RTa | Serinol | DRT | RT | |
| USDA94 | 221 ± 15 | 106 ± 11 | 9.1 ± 3.7 | 14.9 ± 1.7 | 15.8 ± 0.8 | 1.3 ± 0.3 |
| ΔrtxC | 182 ± 21 | 48 ± 6 | <0.1 | 17.6 ± 1.8 | 11.2 ± 0.6 | <0.05 |
| Δrtx::Ω1 | <3.2 | <2.7 | <0.1 | <0.07 | <0.5 | <0.05 |
| ΔrtxC(pLAFRSS) | 88 ± 28 | 62 ± 11 | <0.1 | ND | ND | ND |
| ΔrtxC(pLAFRSS-rtxC) | 107 ± 29 | 33 ± 9 | 1.6 ± 1.1 | ND | ND | ND |
| ΔrtxC(pLAFRSS-PaphrtxC) | 134 ± 34 | 27 ± 13 | 3.5 ± 1.5 | ND | ND | ND |
a
DRT, dihydrorhizobitoxine; RT, rhizobitoxine.
b
Data are means ± SD; ND, not determined.