Figure 1. Generation and characterization of HOL transgenic mice.

(A) Schematic representation of HRE/ODD-luciferase transgene. The promoter contains 5 tandem repeats of the hypoxia responsive element (HRE) of human vascular endothelial growth factor gene. The reporter gene encodes a fusion protein consisting of the nuclear localization signal (NLS) of the simian vacuolating virus 40 T-antigen, the oxygen-dependent degradation domain (ODD) of human HIF-1α (548–603) and the firefly luciferase. (B) FVB/HOL and BALB/HOL mice were intraperitoneally injected with 3,4,5-trihydroxybenzoic acid propyl ester (PG). The bioluminescence imaging was performed 4 h after PG injection. The experiment was repeated three times, and the representative data are shown. (C) Southern blot analysis of the PstI-digested genome of the BALB/c, BALB/HOL, FVB/N, and FVB/HOL mice. The blot was probed with the reporter cDNA fragment of the transgene.