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. 2011 Nov 10;7(11):e1002362. doi: 10.1371/journal.ppat.1002362

Figure 2. U21 reroutes ULBP1 and MICB to a punctate perinuclear compartment.

Figure 2

Left panels: U373 cells (A), and U373 cells expressing each NK ligand and control vector were immunolabeled with antibodies directed against properly-folded class I MHC molecules (W6/32)(A), or against ULBP1 (m295)(D), ULBP2 (G), ULBP3 (J), MICA (M), or MICB (m360) (P), as noted. Middle and right panels: Cells expressing each NK-ligand and U21, as indicated, were double-labeled with antibodies directed against class I MHC molecules (B), ULBP1 (E), ULBP2 (H), ULBP3 (K), MICA (N), or MICB (Q) and anti-U21 (panels C,F,I,L,O, and R). Asterisks in panels E and F denote cells expressing U21 (F) that also exhibit relocalization of ULBP1. Asterisks in panels N, O, Q, and R indicate cells expressing U21 (O and R) that also exhibit reduced labeling of MICA or MICB. The arrowheads in panels Q and R denote a cell with low apparent levels of U21 (R) and normal surface expression of MICB.