Figure 7. Binding of macrophage galactose-type lectin (MGL) to the galactosaminogalactan of A. fumigatus.

ELISA-inhibition of MGL-Fc interacting with α-GalNAc-conjugated polyacrylamide (GalNAc-PAA) by free GalNAc monosaccharides and a mixture of oligosaccharides exclusively composed of N-acetylgalactosamine with an average degree of polymerisation of 7.5 (G25-I). ELISA plates were coated with GalNAc-PAA (2 µg/ml). Plates were blocked with 1% BSA and the recombinant MGL-Fc chimera was added (0.5 µg/ml) for 2 h at room temperature in the presence of 0.125 and 0.25 mM of SGG hydrolysate fractions (G25-I). Binding was detected using a peroxidase-labeled anti-human IgG-Fc antibody.