Figure 7. BNRF1 promotes viral gene expression.

(A) Primary B cells were infected with virus produced from △BNRF1 mutant bacmids either complemented with empty vector, wild type BNRF1 or the d26 Daxx-interaction-deficient mutant BNRF1. Virus infected cells were subject to reverse transcription PCR assay of viral gene expression for EBNA1, EBNA2, or BZLF1. (B) 293HEK cells were co-transfected with wild type EBV genomes and either empty vector, WT BNRF1 or the d26 Daxx-interaction-deficient mutant BNRF1. Transfected cells were subject to reverse transcription PCR assay of viral gene expression for EBNA1, EBNA2, EBNA3C, or BZLF1.