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The nucleotide and amino acid substitutions at dhfr gene screened for by PCR-SSOP
| Codon | 50 | 51 | 59 | 108 | 164 |
|---|---|---|---|---|---|
| Wild type | Cys (C) TGT |
Asn (N) AAT AAC |
Cys (C) TGT |
Ser (S) AGC | Ile (I) ATA |
| Mutant |
Arg (R) CGT |
Ile (I) ATT |
Arg (R) CGT |
Asn (N) AAC Thr (T) ACC |
Leu (L) TTA |
