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. 2011 Oct 11;7:33. doi: 10.1186/1746-4811-7-33

Figure 2.

Figure 2

DNA analyses of randomly picked colonies showing copy number increases of the composite vectors pHZAUBAC1 and pHZAUBIBAC1. A. DNA analysis of randomly picked colonies transformed with pIndigoBac536 (lane 10), pIndigoBac536-S (lane 9) and the ligation product of pIndigoBac536-S and pGEM-4Z at the HindIII cloning site (pHZAUBAC1, lanes 1-8). DNA samples were prepared from 2 mL overnight cultures and were resuspended in 50 μL TE. Aliquots of 5 μL were digested with HindIII and were loaded onto a 1% agarose gel. pIndigoBAC536-S is 470 bp smaller than pIndigoBAC536. B. DNA analysis of randomly picked colonies transformed with BIBAC-S (lane 9) and the ligation product of BIBAC-S and pGEM-4Z at the BamHI cloning site (pHZAUBIBAC1, lanes 1-8). DNA samples were prepared from 2 mL overnight cultures and were resuspended in 50 μL of TE. Aliquots of 5 μL were digested with BamHI and were loaded onto a 1% agarose gel. The marker is lambda/HindIII.