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Stability test for maize B73 BIBAC clones in Agrobacterium: direct method. DNA samples from the maize B73 BIBAC clone B6 that contains an insert of 160 kb (lane 1; see also Figure 7) was transferred into Agrobacterium EHA105, and randomly selected colonies (lanes 2 to 38) were cultured for 48 h at 28°C. Plasmids were isolated from each culture, were digested with I-SceI and were separated on CHEF gels.
