Table 5. Candidate reference genes ranked in order of their stability throughout TS cell differentiation using the NormFinder, GENorm and BestKeepr software tools.
NormFinder | geNORM | BestKeeper | |||||||
Rank | Gene Name | Stability Value | Rank | Gene Name | Stability Value | Rank | Gene Name | ||
1 | Ywhaz | 0.095 | 1 | Ywhaz | 0.018 | 1 | Ywhaz | ||
2 | Pgk1 | 0.096 | 2 | Pgk1 | 0.029 | 2 | Pgk1 | ||
3 | H2afz | 0.102 | 3 | Hk2 | 0.036 | 3 | Hk2 | ||
4 | Hk2 | 0.104 | 4 | H2afz | 0.04 | 4 | H2afz | ||
5 | Hprt | 0.11 | 5 | Hprt | 0.042 | 5 | Hprt | ||
6 | Tbp | 0.111 | 6 | Tbp | 0.044 | 6 | Tbp | ||
7 | Actb | 0.112 | 7 | Actb | 0.044 | 7 | Sdha | ||
8 | Sdha | 0.122 | 8 | Ppia | 0.048 | 8 | Actb | ||
9 | Hsp70 | 0.127 | 9 | Sdha | 0.06 | 9 | Ppia | ||
10 | Ppia | 0.128 | 10 | Hsp70 | 0.061 | 10 | Hsp70 | ||
11 | Gusb | 0.133 | 11 | Gusb | 0.065 | 11 | Gusb | ||
12 | Gapdh | 0.153 | 12 | Gapdh | 0.093 | 12 | Gapdh | ||
13 | B2m | 0.183 | 13 | B2m | 0.12 | 13 | B2m | ||
14 | Rn7sk | 0.202 | 14 | Rn7sk | 0.24 | 14 | Rn7sk |