Figure 5. MSI5 and FVE Are Required for De Novo Silencing of the FWA Transgene.

(A) Flowering times of flc and flc;msi5-2;fve mutants transformed with an FWA transgene. Total leaf number for each line or T₁ transgenic population with 15–16 plants grown in LD, were scored. Bars indicate SD. (B) Cytosine methylation at the tandem repeats of FWA transgene (in T₁ transformants) determined by bisulfite sequencing. For each genotype, 15 plasmids derived from bisulfite-converted genomic DNA were sequenced and examined.