Table 1.
Nuclear ribosomal and mitochondrial DNA haplotype code identification for lymnaeid species and populations studied from Venezuela
| Lymnaeid species | Populations | Preliminary classification | rDNA ITS-2 | rDNA ITS-1 | mtDNA cox1 | Combined H nomenclature | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Locality (State) | Coordinates and altitude | Habitat | No. snails (month of collection) | H | Acc. No. | H | Acc. No. | H** | Acc. No. | |||
| L. cubensis | Magdaleno, Lago Valencia-Maracay (Aragua) | 10°07'36" N 67°35'42" W 428 m | water canal Calicanto flowing into the large Lake of Valencia-Maracay | 2 (November) | L. cubensis *** | 4* | GenBank: F514088 | B | EMBL: FN182202 | b | EMBL: FN182205 | L.cub-4B, cox1b |
| Mucura, Lago Valencia-Maracay (Aragua) | 10°06'38" N; 67°28'47" W 410 m | pool of stagnant water close to the large Lake of Valencia-Maracay | 5 (November) | L. cubensis *** | 4* | GenBank: JF514088 | B | EMBL: FN182202 | b | EMBL: FN182205 | L.cub-4B, cox1b | |
| El Joque, Jají (Mérida) | 8°35'10" N; 71°20'36" W 1995 m | irrigation canal in dairy cattle farm | 40 (June) | Lymnaea sp. aff. cubensis *** | 2 | EMBL: FN182200 | B | EMBL: FN182202 | b | EMBL: FN182205 | L.cub-2B, cox1b | |
| Estanques Lagunillas (Mérida) | °30'15" N; 71°23'41" W 1032 m | connecting canal from permanent pond inside urban area | 15 (March) | L. cubensis | 4* | GenBank: JF514088 | B | EMBL: FN182202 | b | EMBL: FN182205 | L.cub-4B, cox1b | |
| L. neotropica | La Linda, Güigüe (Carabobo) | 10°04'53" N; 67°47'15" W 450 m | water canal at the southern part of the Lake of Valencia-Maracay | 3 (May) | L. cubensis *** | 2* | GenBank: JF514089 | A | EMBL: AM412228 | c* | GenBank: JF461485 | L.neo-2A, cox1c |
| Finca El Arenal, Tucacas (Falcón) | 10°43'29" N; 68°23'25" W 30 m | irrigation canals from Aroa river in Parcelas Agrotécnicas of a farm, in the way to Las Lapas | 82 (May) | L. cubensis *** | 2* | GenBank: JF514089 | A | EMBL: AM412228 | d* | GenBank: JF461486 | L.neo-2A, cox1d | |
| G. truncatula | Iglesia Monterrey (Mérida) | 8°38'39" N; 71°07'24" W 1900 m | irrigation canal along pasture grassland | 47 (March) | Galba/Fossaria sp. | 2 | EMBL: AJ296271 | D* | GenBank: JF514090 | b* | GenBank: JF461487 | G.tru-2D, cox1b |
| San Rafael, Mucuchies A (Mérida) | 8°44'42" N; 70°55'23" W 3150 m | grassland with slight slope flooded from small stream | 250 (March) | L. viatrix | 2 | EMBL: AJ296271 | D* | GenBank: JF514090 | b* | GenBank: JF461487 | G.tru-2D, cox1b | |
| San Rafael, Mucuchies B (Mérida) | 8°44'46" N; 70°55'21" W 3200 m | small stream south from the village | 225 (March) | Galba/Fossaria sp. | 2 | EMBL: AJ296271 | D* | GenBank: JF514090 | b* | GenBank: JF461487 | G.tru-2D, cox1b | |
| San Isidro, Apartaderos (Mérida) | 8°47'48" N; 70°51'33" W 3500 m | small stream behind the school of children San Isidro | 154 (March) | L. cubensis | 2 | EMBL: AJ296271 | D* | GenBank: JF514090 | b* | GenBank: JF461487 | G.tru-2D, cox1b | |
| Paso del Cóndor (Mérida) | 8°50'41" N; 70°48'41" W 4080 m | road gutter with water descending from mountain fountain | 7 (March) | Galba/Fossaria sp. | 2 | EMBL: AJ296271 | D* | GenBank: JF514090 | b* | GenBank: JF461487 | G.tru-2D, cox1b | |
| L. schirazensis | Hotel Valle Grande (Mérida) | 8°40'28" N; 71°06'03" W 2200 m | small gutter besides external wall of hotel building | 215 (March) | Galba/Fossaria sp. | 1 | GenBank: JF272601 | A | GenBank: JF272603 | a | GenBank: JF272607 | L.schi-1A, cox1a |
| Laguna Fe y Alegría (Mérida) | 8°37'29" N; 70°49'33" W 1840 m | wild grass and stones besides small stream of fast running water | 69 (March) | L. cubensis | 1 | GenBank: JF272601 | A | GenBank: JF272603 | a | GenBank: JF272607 | L.schi-1A, cox1a | |
| La Trampa (Mérida) | 8°32'25" N; 71°27'23" W 2170 m | besides small water collection on hillside | 12 (May) | G. truncatula *** | 1 | GenBank: JF272601 | A | GenBank: JF272603 | a | GenBank: JF272607 | L.schi-1A, cox1a | |
| L. meridensis | Laguna Mucubaji (Mérida) | 8°47'52" N; 70°49'32" W 3550 m | surroundings and shore of large natural pool | 29 (October) | L. cousini | 1 | EMBL: FN598154 | A | EMBL: FN598159 | a | EMBL: FN598164 | L.mer-1A, cox1a |
| P. columella | La Linda, Güigüe (Carabobo) | 10°04'53" N; 67°47'15" W 450 m | water canal at the southern part of the Lake of Valencia-Maracay | 2 (May) | P. columella *** | 2 | EMBL: FN598156 | A | EMBL: FN598160 | a | EMBL: FN598165 | P.col-2A, cox1a |
| El Valle (Mérida) | 8°38'41" N; 71°07'24" W 1930 m | irrigation canal along pasture grassland close to Iglesia Monterrey | 4 (March) | Lymnaeidae gen. sp. | 1 | EMBL: FN598155 | A | EMBL: FN598160 | a | EMBL: FN598165 | P.col-1A, cox1a | |
H = haplotype; * = new haplotypes for the corresponding lymnaeid species; ** = only preliminary haplotypes due to incomplete gene sequence; *** = material collected, alcohol-fixed, preliminarily classified and furnished by colleagues