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The in-vitro PZase assay consisted of four steps. (A) Extraction of DNA from samples. (B) PCR amplification of the pncA gene. (C) In-vitro synthesis of PZase. (D) Assay of PZase activity. T7 P: T7 promoter. 5′UTR: 5′untranslated regulatory regions containing translational enhancer elements.
