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. Author manuscript; available in PMC: 2012 Dec 1.
Published in final edited form as: J Virol Methods. 2011 Sep 17;178(1-2):143–152. doi: 10.1016/j.jviromet.2011.09.004

Table 5.

Results of recombinant viral protein and viral lysate protein based MMIAs against a challenge panel of sera/plasma compared to consensus results from 12 different laboratories using an unspecified variety of reagents and methods

Sample ID
187096 188397 188398 188399 188400 188402 188451 188452 184261 188722 188800 188801 188804 188807 188808 188809 Hit ratio
B virus Consensus Results a N P P P N P* N N N N P P P* N N N
B virus gD (VTI) b - - - - - N - - - - - - N - - - 14/16
HVP2 (CRL) c - - - - - - - - - - - - - - - - 16/16
HVP2 (UCD) d - - - - - N - - - - - - - - - - 15/16
STLV Consensus Results a N N N P N P N N N N N P N P P P
STLV-1 gag (VTI) b - - - - - - - - P - - - - - - - 15/16
STLV (CRL) c - - - - - - - - - - - - - - - - 16/16
STLV (UCD) d - - - - - - - - - - - - P - - - 15/16
SIV Consensus Results a N N N N N N N N N P N N N N N N
SIV gag (VTI) b - - - - - - - - P - - - - - - - 15/16
SIV (CRL) c - - - - - - - - - - - - - - - - 16/16
SIV env (CRL) e - - - - - - - - - - - - - - - - 16/16
SIV (UCD) d - - - - - - - - - - - - P - - - 15/16
SFV Consensus Results a P P P P P P N N P P P P P P P P
SFV-1 gag (VTI) b - - - - - - - - - - - - - - - - 16/16
SFV (CRL) c - - - - - - - - - - - - - - - - 16/16
SFV (UCD) d - - - - - - - - N - - - - - - - 15/16
SRV Consensus Results a P* P P P* P P N N N N N N N N* N N
SRV 4/5 gag (VTI) b - - N - - - - - - - - - - - - - 15/16
SRV (CRL) c N N N N N - - - - - - - - - - - 11/16
SRV env (CRL) e - - - N - - - - - - - - - P - - 14/16
SRV (UCD) d N - N N N N - - - - - - P P - P 8/16
a

consensus results from 12 laboratories;

b

MMIAs using recombinant virus proteins from Vaccine Technology Inc. (VTI);

c

MMIAs using virus lysate preparation from Charles River Laboratory (CRL);

d

MMIAs using virus proteins from University of California, Davis Clinical Proteomics Core Laboratory (UCD);

e

MMIA using recombinant viral envelope proteins from Charles River Laboratory (CRL);

*

more than two laboratories did not agree with the consensus result; P: positive; N: negative; dash results agree with the consensus result.