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. 2011 Aug 15;124(16):2837–2850. doi: 10.1242/jcs.081968

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© 2011.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial Share Alike License (http://creativecommons.org/licenses/by-nc-sa/3.0), which permits unrestricted non-commercial use, distribution and reproduction in any medium provided that the original work is properly cited and all further distributions of the work or adaptation are subject to the same Creative Commons License terms.

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Fig. 1. — Characterization of Rad18 KD mice. (A) Rad18 mRNA expression in testis, kidney, brain, thymus, spleen and liver of three control mice and three Rad18 KD mice. Error bars indicate s.e.m. values. (B,C) RAD18 expression in total cell extracts (20 μg) from testis of 4-week-old (B) and 19-week-old (C) from control (ctr) and Rad18 KD was detected on immunoblots. β-tubulin was used as loading control. (D) Average litter size obtained from matings between control and wild type (ctr), knockdown and wild type (kd), and two knockdowns (kd×kd). Error bars indicate s.e.m. values. (E,F) Body, testis and epididymis weights (E,F) and the number of sperm (F) from 4-week-old (E) and 19-week-old (F) control and Rad18 KD mice. Error bars indicate s.e.m. *P<0.05 and **P<0.01 (Mann–Whitney U-test). Blue and red bars indicate control and Rad18 KD, respectively.