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. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Mol Cancer Ther. 2011 Sep 2;10(11):2146–2156. doi: 10.1158/1535-7163.MCT-11-0399

Figure 4. DiFiD inhibits Notch-1 activation and the γ-secretase complex.

Figure 4

A, Real Time PCR analysis of total RNA from MiaPaCa-2 cells treated with 1 µM DiFiD treatment for 24 h. DiFiD treatment showed reduction in the expression of Notch-1, its ligand Jagged-1and the downstream target gene Hes-1 (*P <0.05). Data from three independent experiments. B, Lysates were also analyzed by western blotting. DiFiD treatment showed significant reduction in the expression of cleaved Notch-1, its ligand Jagged-1 and its target genes Hes-1 and Hey-1. C, MiaPaCa-2 cells treated with 1 µM of DiFiD for 24 h were subjected to immunoflurescence staining using anti-Notch-1, anti-Jagged-1 and anti-Hes-1 antibodies. DiFiD treatment resulted in reduced levels of Notch-1 protein in the nucleus, reduced levels of membrane bound Jagged-1 and inhibited Hes-1 expression (600X). D, γ-secretase complex proteins such as Presenilin-1 and Nicastrin were measured after treatment with 1 µM DiFiD for 24 h. Both the mRNA and protein levels were reduced with DiFiD treatment (*P <0.05). Data from three independent experiments.