Table 1.

Boundaries of Safe, Twilight and Dark Zones used by NPS-HomPPI^a.

	log(EVal)	≤ -100
Safe Zone	Positive Score	≥80%
	log(LAL)	≥5.2
	log(EVal)	≤ -50
Twilight Zone 1b	Positive Score	≥65%
	log(LAL)	≥4
	log(EVal)	≤ 1
Twilight Zone 2b	Positive Score	≥60%
	log(LAL)	≥4
	log(EVal)	≤ 1
Dark Zone	Positive Score	≥0
	log(LAL)	≥0

^a Thresholds were chosen based on interface conservation analysis of proteins in Trans135.

^b Twilight Zone is divided into two sub-zones: Twilight Zone 1 with stricter thresholds, and Twilight Zone 2 with looser thresholds.