Skip to main content
. Author manuscript; available in PMC: 2012 Sep 15.
Published in final edited form as: Oncogene. 2011 Aug 8;31(11):1431–1441. doi: 10.1038/onc.2011.332

Figure 3. Silencing of endogenous CHKA reduces EGF-stimulated growth of MCF-7 and MCF-10A cells.

Figure 3

Cells were transfected with control (siCTRL) or CHKA (siCHKA) siRNA and treated as described in Materials and Methods. A. After starvation, MCF-7 cells were incubated with or without 10 ng/mL EGF, and cell viability was measured by the alamarBlue assay (n=3). B. Growth of MCF-10A cells in response to 5 ng/mL EGF was monitored by cell counting (n=3). Similar results were observed using the MTS assay (data not shown). Statistical significance between asterisk marked treatment and other treatments was determined by Student's t test. *: p < 0.05, **: p <0.01. The results are expressed as the mean ± SEM for three experiments. C, D. The same siRNA transfected samples in A and B were harvested at different time points of EGF stimulation and Western blotted to assess levels of CHKA along with control GAPDH. C: MCF-7. D: MCF-10A.