Skip to main content
. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Mech Dev. 2011 Jul 23;128(7-10):359–367. doi: 10.1016/j.mod.2011.07.002

Figure 1.

Figure 1

A: Isolated intact E11.5 ureteric buds (iUB) express Ret (300 bp), but not GDNF (291 bp), mRNA. E12.5 mouse kidneys (Kid) express both Ret and GDNF mRNA. B and C: iUB, immortalized UB cells (UBc) and E12.5 mouse kidneys express angiotensin (Ang) II AT1R and AT2R mRNA. D: Effect of Ang II on branching of iUBs. iUBs were dissected on E11.5 and grown in collagen matrix gels in the presence of GDNF, FGF1 and media (control) or GDNF, FGF1 and Ang II (10−5 M) for 48 hours. Numbers below each iUB image show number of UB tips (marked with arrows) at each time point. E: Bar graph shows the effect of treatments on the number of UB tips after 48 hours of culture. The number of iUB tips in higher in Ang II-treated iUBs compared with media control (Mean±SEM).