FIG. 1.

Nucleotide and amino acid sequences of the N terminus of the bovine TLR9-encoding gene. (A) 5′-Terminal sequence of the TLR9-encoding gene. The sequence is from BAC clone 820 and is also shown as retrieved from 5′-RACE clones (bold face letters). The most 5′-located nucleotides, as retrieved from the longest 5′-RACE clones, from various tissues are specified (*, mammary gland; §, brain; #, macrophages). Two SP-1 boxes are located in the presumed promoter sequence. An intron (splice donor and acceptor dinucleotides are underlined) separates the translational start codon from the rest of the coding sequence (codons are indicated). The sequence around the 5′ splice donor dinucleotide is precisely conserved between cattle, humans, and mice (boxed). (B) Comparison of the N-terminal amino acid sequence of TLR9 factors. The bovine signal peptide is shorter by 1 aa residue than those from humans and mice (:, identical residues; ., equivalent amino acid residues).