FIG.6.

BNBD5 is expressed in MEC and cells lining the milk ducts. Mammary gland sections of an udder were hybridized with either the antisense probe (A to C) or the sense control (D) of the BNBD5-encoding gene. The signal was developed for 20 h. The reddish label is found in MEC lining the milk ducts and in collapsed alveoli, and it is in its highest concentrations at basolateral locations inside the cells. The samples were taken from a slaughtered cow which had not been milked for 2 weeks, due to the infection. Parallel samples revealed high levels of BNBD5 in real-time RT-PCR quantification. Magnification, ×40 (A) and ×200 (B to D).