Table 3.
Purification step | Total protein (mg) | Total activity (U) | Specific activity (U/mg) | Purification (fold) | Yield (%) |
---|---|---|---|---|---|
Culture filtrate | 1,582 | 12,462 | 7.877 | 1.0 | 100 |
(NH4)2SO4 precipitate | 106 | 7,341 | 69.25 | 8.71 | 58.90 |
DEAE cellulose | 64 | 4,896 | 76.5 | 9.68 | 39.28 |
Sephadex G 50 | 25 | 2,038 | 81.52 | 10.34 | 16.35 |